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目的:分析灯盏花乙素(SCU)在大鼠体外震波(CSWT)中的作用机制。方法:随机将35只大鼠分为A(梗死对照组)、B(CSWT组)、C(FAK抑制+CSWT组)、D(IBTX+CSWT组)、E(SCU高剂量+CSWT组)、F(SCU中剂量+CSWT组)、G(SCU低剂量+CSWT组)7组,各5只,给予对应药物及体外震波处理后,取心脏组织,采用Real time-PCR法检测各组血管内皮生长因子A(VEGF-A)、整合素β1(ITGB1)、单核细胞趋化因子1(MCP-1)、内皮一氧化氮合成酶(eNOS)基因表达情况,采用蛋白印迹法(Western Blot)检测各组VEGF、MCP-1、eNOS、CD29蛋白的表达。结果:1与A组比较,各组VEGF-A、ITGB1均上升,B、E组MCP-1下降,C、D、G组MCP-1下降,B、F、G组eNOS基因表达降低,C、D、E组基因表达上升(P<0.05);与B组对比,C、E、F、G组VEGF-A基因表达均上升,C、D组ITGB1基因表达降低,E、F、G组ITGB1基因表达上升,C、D、E、G组MCP-1降低,E组上升,C、E组eNOS上升,D、F、G组下降(P<0.05);2与A组比较,B、C组VEGF蛋白表达上升,D、E、F、G组下降;各组MCP-1蛋白表达均上升;除E组eNOS表达降低外,其余各组均上升,C、D、E、F、G组CD29蛋白表达降低(P<0.05)。与B比较,C组VEGF上升,D、E、F、G组均降低(P<0.05),C、F组MCP-1蛋白表达上升,其余各组均下降(P<0.05),除E、G组外,各组eNOS蛋白表达均上升(P<0.05),各组CD29蛋白表达均下降(P<0.05)。结论:SCU联合CSWT可改善心梗大鼠心肌缺氧、缺血状态,促进新生血管形成,有较好的抗氧化应激作用。
Objective: To analyze the mechanism of scutellarin (SCU) in rat in vitro shock wave (CSWT). Methods: 35 rats were randomly divided into three groups: A (infarction control group), B (CSWT group), C (FAK inhibition + CSWT group), D (IBTX + CSWT group), E (SCU high dose + CSWT group) F (SCU medium dose + CSWT group), G (SCU low dose + CSWT group), 7 rats in each group, and 5 rats in each group. After administration of the corresponding drugs and shock wave treatment, heart tissues were obtained. Real time- The expressions of VEGF-A, ITGB1, MCP-1 and eNOS were detected by Western Blot. The expressions of VEGF, MCP-1, eNOS and CD29 in each group were detected. Results: 1 Compared with group A, the levels of VEGF-A and ITGB1 in each group increased, the levels of MCP-1 decreased in groups B and E, the levels of MCP-1 decreased in groups C, D and G, the levels of eNOS decreased in groups B, F and G (P <0.05). Compared with group B, the expression of VEGF-A gene in groups C, E, F and G increased, while the expression of ITGB1 in groups C and D decreased The mRNA expression of ITGB1 increased in group C, D, E and G, but decreased in group C, D, E and G (P <0.05); Group E increased, group C and E increased eNOS, The protein expression of VEGF in group C increased, but decreased in groups D, E, F and G; the expression of MCP-1 increased in all groups; the expression of eNOS in group E increased except C, D, E, F, G Group CD29 protein expression decreased (P <0.05). Compared with group B, the VEGF in group C increased, while in group D, E, F and G decreased (P <0.05); the expression of MCP-1 in group C and group F increased (P <0.05) Except G group, the expression of eNOS protein in each group increased (P <0.05), and the expression of CD29 protein decreased in all groups (P <0.05). Conclusion: Combination of SCU and CSWT can improve myocardial ischemia and myocardial ischemia, promote neovascularization and have good anti-oxidative stress effects.