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目的:在分子水平上对浙产野生白及进行遗传多样性研究,以确定浙产白及生物多样性现状,为有效保护浙产白及种质资源提供理论基础。方法:采用简单重复序列区间(ISSR)标记技术对浙江九个不同产地白及进行研究,通过UPGMA聚类进行分析。结果:从45个ISSR随机引物中筛选出12条引物,共扩增出164条条带,其中多态性条带143条,多态性达87.19%,品种之间的遗传相似系数范围在0.59～0.76间;聚类分析结果显示九个浙产白及分为两大分支:杭州、宁波和德清为一支,淳安、天台、温州、临安、嘉兴、衢州为另一支。结论:浙产白及遗传多样性较低,可引进外来品种或通过杂交育种的方式丰富浙产白及种质资源,并需要对白及种质资源进行有效的保护和合理的利用。
OBJECTIVE: To study the genetic diversity of Zhejiang wild white peony at molecular level to determine the status quo of peanut white and biodiversity, and provide the theoretical basis for the effective protection of white peony and germplasm resources. Methods: The ISSR markers were used to study the genetic diversity of nine white areas in Zhejiang Province and analyzed by UPGMA clustering. Results: Twelve primers were screened out from 45 ISSR random primers. A total of 164 bands were amplified, of which 143 were polymorphic bands with a polymorphism of 87.19%. The genetic similarity coefficients of the two cultivars ranged from 0.59 ~ 0.76. The results of cluster analysis showed that nine Zhejiang white peas were divided into two major branches: Hangzhou, Ningbo and Deqing, with Chun’an, Tiantai, Wenzhou, Lin’an, Jiaxing and Quzhou as the other. Conclusion: The yield and quality of Zhejiang white peony and its genetic diversity are relatively low. It can be used to enrich the white peony and germplasm resources by introduction of alien species or by crossbreeding. The white peony and germplasm resources need to be effectively protected and rationally utilized.