研究牙胚的分化、发育及牙齿硬组织的形成,常用器官培养法,很少用长时间器官培养进行研究。以研究牙齿的发育,特别是确定釉质形成的模型为目的,进行了齿胚长时间培养和组织学观察。胎龄16.5日的小鼠的下颌第一白齿帽状期牙胚,用Trowell 改良法在BGJ_b 培养基中添加胎牛血清、谷氨酰胺、甘氨酸、维生素 To study the differentiation and development of tooth germs and the formation of tooth hard tissues, the common organ culture method is rarely used for long-term organ culture studies. To study the development of teeth, especially for the determination of enamel formation model for the purpose of a long period of tooth germ culture and histological observation. The first white-toothed cap dentate germ of mice of gestational age of 16.5 days was treated with Trowell's modified method by adding fetal bovine serum, glutamine, glycine, vitamine