论文部分内容阅读
目的探讨依达拉奉对帕金森病(PD)小鼠脑细胞自噬的影响。方法随机将30只健康雄性C57BL/6小鼠,分为正常对照组、模型组、治疗组各10只。后两组给予腹腔注射百草枯10 mg/kg、代森锰30 mg/kg,每周二、四各1次,连续6周。建模成功后治疗组给予依达拉奉3 mg/kg腹腔注射2周,正常对照组给予等量生理盐水。观察3组小鼠行为学的改变、并用Western blot法检测中脑自噬特异性标记物LC3Ⅱ、beclin 1的表达。结果与治疗组比较,模型组悬挂时间明显缩短,中脑LC3Ⅱ、beclin 1的表达显著增高。结论依达拉奉治疗可显著抑制PD小鼠模型脑细胞自噬。
Objective To investigate the effect of edaravone on autophagy in brain cells of Parkinson’s disease (PD) mice. Methods Thirty healthy male C57BL / 6 mice were randomly divided into normal control group, model group and treatment group. The latter two groups were given intraperitoneal injection of paraquat 10 mg / kg, thiamine 30 mg / kg every two or four times a week for 6 weeks. After the model was established, the treatment group was given edaravone 3 mg / kg intraperitoneally for 2 weeks, and the normal control group was given the same amount of normal saline. The behavioral changes of the three groups of mice were observed, and the expression of midbrain autophagy-specific markers LC3Ⅱ and beclin 1 were detected by Western blot. Results Compared with the treatment group, the suspension time of the model group was significantly shortened, and the expression of LC3 Ⅱ and beclin 1 in the midbrain was significantly increased. Conclusion Edaravone can significantly inhibit autophagy in PD mouse model.