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以辽鲜1号大豆品种为试材,研究了不同光谱分布对大豆子叶节再生的影响,为菜用大豆子叶节遗传转化受体体系的改进提供借鉴。以萌发7 d的子叶节为外植体,依次接种于丛生芽诱导培养基、伸长培养基和生根培养基中,并且分别置于6种LED组合光源中培育,以荧光灯作为对照。光密度设置为50μmol·m-2·s-1,光周期设置为12h·d-1。结果表明:复合光谱中630 nm红光会抑制丛生芽之间相互伸长,而660 nm红光能显著缓解相互抑制作用,并且更有利于丛生芽形态生长、叶绿素合成和干物质积累以及生根苗形态生长、干物质积累和根系发育。添加绿光或黄光均抑制丛生芽相互之间的伸长,且添加黄光抑制更强,添加绿光可促进丛生芽的叶绿素合成、干物质积累及生根壮苗。
The effects of different spectral distributions on the regeneration of soybean cotyledon sections were studied with Liaoxian No.1 as the test material, which could provide reference for the improvement of the genetic transformation receptor system of soybean cotyledons. The cotyledonary node of 7 d was used as explants, and then inoculated into shoot bud induction medium, elongation medium and rooting medium, respectively, and were cultured in 6 kinds of LED combination light sources. Fluorescent lamps were used as control. The optical density was set to 50 μmol · m-2 · s-1 and the photoperiod was set to 12 h · d-1. The results showed that 630 nm red light could inhibit the elongation of clusters of shoots, while 660 nm red light could significantly relieve mutual inhibition, and more conducive to the growth of clustered buds, the accumulation of chlorophyll and dry matter, Morphological growth, dry matter accumulation and root development. The addition of green or yellow light all inhibited the elongation of the buds, and the addition of yellow light was stronger. The addition of green light promoted the chlorophyll synthesis, dry matter accumulation and rooting of the shoots.