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选用1～3岁龄的国产恒河猴(Macaca mulatta)22只(其中有2只作为对照)进行了戊型肝炎病毒(Hepatitis E Virus简称HEV)的实验感染.第一代感染两批动物:第一批感染4只,有3只发病,第二批感染4只全部发病.而后用第二批感染猴的粪便和肝脏悬液作为第一代,又连续进行传代实验。第二代传代选用的4只猴也全部发病.用肝悬液传代的1只猴也发病.第三代传代选用7只猴,有5只猴发病其中有1只猴(M-34号)是用第二代传代猴的胆汁感染的.所有发病猴血清丙氨酸转氨酶(ALT)水平均有不同程度的升高,一般高于正常值的3～6倍,最高可达10倍左右。肝活检可见肝细胞炎症和坏死。猴的粪便标本用免疫电镜(IEM)检测可见到直径为27～32nm的病毒颗粒。
Twenty-two (1-2) Macaca mulatta domesticated 1- to 3-year-old mice were tested for infection with Hepatitis E Virus (HEV), the first generation of two batches of animals: The first batch of four infected, three onset, the second batch of four infected all the disease and then infected with a second batch of infected feces and liver suspension as the first generation, and continuous passage experiments. The second generation of the passage of the selection of 4 monkeys also all disease.Liver passaged with the passage of a monkey also onset.The third generation of passage selection of seven monkeys, five monkeys, including one monkey (M-34) Is infected with the second generation of chimeric monkey bile.All the serum levels of alanine aminotransferase (ALT) were increased in varying degrees, generally higher than the normal 3 to 6 times, up to 10 times. Liver biopsy shows hepatocellular inflammation and necrosis. Monkey stool specimens with immunoelectron microscopy (IEM) detection can be seen in the diameter of 27 ~ 32nm virus particles.