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本研究的受试者均为21～40岁未患过疟疾的健康志愿者,用的虫株是对氯喹敏感的Tamenie株(埃塞俄比亚)和抗氯喹的Marks株(越南)恶性疟原虫及溪桑株间日症原虫。用的媒介为斯氏按蚊。病人血内配子体数最少为100个/cmm,感染后至少有25%的蚊虫每只含1,000个以上的子孢子。每批蚊虫均在吸取病人血后12～18天内用X线照射,最小的照射剂量为12,000拉德,照射后约60分钟让蚊虫叮咬受试者(免疫),每次叮咬20分钟。同一受试者重复免疫2～8次,每次间隔2周或2周以上。在最后一次叮咬免疫后2周或2周以上,用未照射的感染蚊叮咬攻击。每次都至少有1名对照者接受同
The subjects in this study were all healthy volunteers who did not have malaria between the ages of 21 and 40 years. The strains used were chloroquine-sensitive Tamenie strain (Ethiopia) and chloroquine-resistant Marks strain (Vietnam), P. falciparum Strains of Japanese protozoa. The medium used is An. Sinensis. The patient has at least 100 gametocytes / cmm in the blood and at least 25% of the mosquitoes in the infection contain more than 1,000 sporozoites each. Each batch of mosquitoes were irradiated with X-rays 12 to 18 days after aspiration of the patient’s blood. The minimum irradiation dose was 12,000 rads. The mosquitoes bite the subjects for about 60 minutes after irradiation (immunization), bite each time for 20 minutes. The same subject repeated immunization 2 to 8 times, each interval of 2 weeks or more. Two or more weeks after the last bite immunization, the animals were attacked with uninfected infected mosquito bites. Every time at least one control accepted