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目的建立高效液相色谱(high performance liquid chromatography,HPLC)法测定银龙清热口服液中绿原酸含量的方法。方法色谱柱为Diamonsil C_(18)柱(250 mm×4.6 mm,5μm);流动相为甲醇∶水∶冰醋酸(20∶80∶1);检测波长为324 nm;流速为1.0 ml/min;柱温为35℃。结果在进样量为10μl时,绿原酸浓度在10.0202~125.2525μg/ml,其浓度与峰面积积分值呈良好的线性关系,以绿原酸质量浓度为横坐标X(μg/ml)、峰面积积分值为纵坐标Y进行线性回归,得回归方程为Y=29234X-3971.5,R~2=0.9994;平均加样回收率(n=9)为100.41%,相对标准偏差(relative standard deviation,RSD)为2.70%。结论 HPLC法测定银龙清热口服液中绿原酸的含量结果准确、稳定、重现性好,且简便易行,可用于该制剂中绿原酸的含量测定。
Objective To establish a method for the determination of chlorogenic acid in Yinlong Qingre Oral Liquid by high performance liquid chromatography (HPLC). The column was Diamonsil C 18 column (250 mm × 4.6 mm, 5 μm). The mobile phase was methanol: water: glacial acetic acid (20:80:1). The detection wavelength was 324 nm. The flow rate was 1.0 ml / min. The column temperature was 35 ° C. Results When the injection volume was 10μl, the concentration of chlorogenic acid was in the range of 10.0202 ~ 125.2525μg / ml. The concentration of chlorogenic acid showed a good linear relationship with the integral of peak area. Taking the concentration of chlorogenic acid as X (μg / ml) The regression equation was Y = 29234X-3971.5, R ~ 2 = 0.9994. The average recovery was n = 9 (100.41%). The relative standard deviation RSD) is 2.70%. Conclusion The determination of chlorogenic acid in Yinlong Qingre Oral Liquid by HPLC is accurate, stable, reproducible and simple and can be used for the determination of chlorogenic acid in this preparation.