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目的 研究长期不进展者的病毒生物学特征及其与疾病发展的关系。方法 用感染者和正常人外周血单核细胞(PBMC)共培养的方法分离病毒,终点以共培养上清液中的p24抗原作为病毒生长的评价指标。结果 (1)从15例长期不进展者分离到4株病毒,占26.7％。同时取该地区HIV-1感染进展者血样20份,分离到14株病毒,阳性率为70.0％;(2)病毒分离率影响因素的试验结果表明,病毒的分离率与CD_4细胞数有明显关系,CD_4细胞数越高,分离率越低。反之,CD_4细胞数越低,病毒分离率越高。此外,去除CD_8细胞后可以显著提高病毒的分离率;(3)病毒生物学特性观察发现长期不进展者分离株均属生长缓慢、低滴度、非致细胞融合型(NSI型),在T细胞不能生长的病毒;(4)大部分进展者分离株与长期不进展者分离株在生物学特性方面没有显示区别。结论 长期不进展者与大部分进展者的病毒生物学特征没有明显区别,因此,推测病毒学因素可能不是决定病程进展的最主要因素。影响疾病进展的其它因素,如免疫因素等尚需进一步研究。
Objective To study the characteristics of virus biology and its relationship with disease development in long-term non-progressors. Methods Virus was co-cultured with peripheral blood mononuclear cells (PBMCs) of infected and normal people. The end point of the co-culture supernatants p24 antigen as an indicator of virus growth. Results (1) Four viruses were isolated from 15 cases of long-term non-progression, accounting for 26.7%. At the same time taking 20 blood samples from HIV-1 infected persons in this area, 14 viruses were isolated and the positive rate was 70.0%. (2) The experimental results of virus isolation rate showed that the virus isolation rate was significantly related to the number of CD_4 cells , The higher the number of CD_4 cells, the lower the isolation rate. Conversely, the lower the number of CD_4 cells, the higher the rate of virus isolation. In addition, the removal of CD_8 cells can significantly improve the isolation rate of the virus; (3) the biological characteristics of the virus observed in the long-term non-progressing isolates are slow growth, low titer, non-cell fusion (NSI type) Cells can not grow the virus; (4) most of the progenitor isolates and long-term non-progressed isolates in the biological characteristics of no difference. Conclusions There is no significant difference in the biological characteristics of most patients with long-term non-progression. Therefore, it is suggested that virological factors may not be the most important factor in the progression of the disease. Other factors affecting the progress of the disease, such as immune factors need further study.