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观察了神经节苷酯对急性低氧组大鼠（Ａ组：模拟海拔７００ｍ，５ｈ；Ｂ组：给神经节苷酯）和常氧对照组大鼠（Ｃ组：常氧；Ｄ组：给神经节苷酯）脑组织Ｃａ￣（２＋）、钙调素（ＣａＭ）及ＣａＭ依赖性蛋白激酶Ⅱ（ＣａＭＰＫⅡ）活性的影响。用Ｆｕｒａ－２测得Ｃａ￣（２＋）水平分别为７２２．１±８１．２．６４０．６±７４．４、２５８。７±３９．２和２６３．８±４１．３ｎｍｏｌ／Ｌ，Ａ组明显高于常氧对照组（Ｐ＜０．０１）；Ｂ组明显低于Ａ组（Ｐ＜＜０．０５）。用流式细胞仪（ＦＡＣＳ）所测ＣａＭ平均荧光强度分别为４０．２±４．７、４４．３±４．８．４６．１±５．１和４５．９±５．０道；Ａ组明显低于常氧对照组。用同位素（ｒ￣（３２）－ＡＴＰ）液闪计数法所测ＣａＭＰＫⅡ活性分别为１８２．９±７．９、１９２．３±８．２、１９７．９±９．２和１９８．２±９．３ｐｍｏｌｐｉ．ｍｉｎ￣（－１）ｍｇ￣（－１）ｐｒｏｔｅｉｎ，Ａ组明显低于其它三组，急性低氧Ｂ组与常氧对照组比无明显差别。表明，低氧时神经节苷酯可以减少胞内Ｃａ￣（２＋）蓄积和稳定ＣａＭ和ＣａＭＫｉｎａｓｅⅡ的变化．
The effects of ganglioside on acute hypoxia rats (group A: simulated altitude 700 m, 5 h; group B: ganglioside) and normoxia control group (group C: normoxia; group D: Ganglioside) on the activity of Ca 2+, CaM and CaMPⅡ in brain tissue. The levels of Ca 2+ in Fura-2 were 722.1 ± 81.2.640.6 ± 74.4, 258.7 ± 39.2 and 263.8 ± 41.3 nmol / L, respectively, and group A Which was significantly higher than that of normoxia control group (P <0.01); B group was significantly lower than that of A group (P <0.05). The average fluorescence intensity of CaM measured by flow cytometry (FACS) was 40.2 ± 4.7, 44.3 ± 4.8.46.1 ± 5.1 and 45.9 ± 5.0, respectively; A Group was significantly lower than the normoxic control group. The CaMPKII activities measured by isotope (r ~ (32) -ATP) liquid scintillation counting were 182.9 ± 7.9, 192.3 ± 8.2, 197.9 ± 9.2 and 198.2 ± 9, respectively .3pmolpi. min ~ (-1) mg ~ (-1) protein, A group was significantly lower than the other three groups, acute hypoxia B group and normoxic control group no significant difference. The results showed that ganglioside decreased the intracellular Ca 2+ accumulation and stabilized the changes of CaM and CaMKinase Ⅱ during hypoxia.