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[目的 ]探讨慢性乙型肝炎病毒C区基因突变株对干扰素治疗的应答反应 .[方法 ]对 5 8例慢性乙型肝炎患者用重组干扰素 α(总用量 5 2 2× 10 6U)治疗 .前C区基因停止密码 2 8的突变株由限定酶断片长短的多形性测定 .C区基因的变异是用增殖各患者的 5种乙型肝炎病毒DNA克隆的方法检测 .[结果 ]干扰素疗程结束 6个月后 ,前C区变异株 2 3例中 14例 (6 0 % )乙型肝炎病毒e抗原阴转 ,无变异的野生型 35例中 15例 (4 3 % )乙型肝炎病毒e抗原阴转 ,二组无显著性差异 ,而前C区野生型 35例中C区基因 5个克隆全变异组 (2 4例 )的乙型肝炎病毒e抗原阴转率高于C区基因 5克隆中至少 1个克隆未变异组 (11例 ) (5 8%与 9% ) .[结论 ]感染乙型肝炎病毒变异株的乙型肝炎病毒e抗原阳性患者对干扰素应答良好 ,但这种变异株并不一定非在前C区 ,而C区基因的变异株亦可通过干扰乙型肝炎病毒e抗原的合成和分泌 ,从而影响干扰素的应答反应
[Objective] To investigate the response of C gene mutation in chronic hepatitis B virus to interferon therapy. [Methods] Fifty-eight patients with chronic hepatitis B were treated with recombinant interferon α (522 × 10 6 U) The mutations in the precordial C-gene stop codon 28 were determined by the polymorphism that defined the length of the enzyme fragment, and the mutation in the C-region gene was detected by DNA-cloning of 5 Hepatitis B viruses in each of the proliferating patients. [Results] Six months after the end of the course of treatment, 14 (60%) of the 23 cases of pre-C-region mutations were negative for hepatitis B virus e antigen and 15 cases (43%) of 35 cases of wild-type without mutation Hepatitis e e antigen turned negatively, there was no significant difference between the two groups. However, the negative conversion rate of hepatitis B virus e antigen was higher in C group (5 cases) than in C group (35 cases) At least one clonal non-mutated group (11 cases) (5 8% and 9%) was found in 5 clones of district genes. [Conclusion] HBeAg-positive patients infected with hepatitis B virus have a good response to interferon, However, this variant is not necessarily in the pre-C region, while the C-region gene variant may also interfere with the hepatitis B virus e antigen Synthesis and secretion, thus affecting the IFN responses