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目的 实验观察不同扩张时间皮肤微血管系及其血流动力学的变化规律。方法 以兔为实验动物 ,用激光多普勒微循环显微成像、酶组织化学染色并计算机图像分析等形态学研究手段 ,观测正常及不同扩张时间皮肤血液流速、流量等血流动力学指标及管径、密度等微血管形态学指标。结果 对照组、扩张组在微血管直径、血流速度、血流量及微血管密度方面存在显著差异 (P<0 .0 1) ,直径、流量测量值从第 1～ 3周分别从 11.16± 1.0 1μm,(11.15± 0 .85 )×10 5 μm3增至 13.91± 1.4μm,(15 .86± 0 .93)× 10 5 μm3,微血管密度在第 1～ 4周从 0 .0 2 2± 0 .0 2μm2 /μm2 增至 0 .0 6 1± 0 .0 0 3μm2 /μm2 ,随扩张时间延长上述观测值在出现一过性下降后又呈现同样变化。流速在扩张后增大并维持平均 10 12 .45 μm/s的较高水平。结论 扩张皮肤约 3～ 4周完成一个微血管增生构筑演变周期 ,达到一次直径、流量及密度最大值。
Objective To observe the changes of the skin microvessel system and its hemodynamics at different time of expansion. Methods Rabbits were used as experimental animals. The hemodynamic indexes such as blood flow velocity and flow rate were observed by laser Doppler microcycle microscopy, enzyme histochemical staining and computer image analysis. Diameter, density and other microvascular morphological indicators. Results There were significant differences in microvessel diameter, blood flow velocity, blood flow and microvessel density between the control group and the dilatation group (P <0.01), and the diameter and flow rate measurement ranged from 11.16 ± 1.0 1μm, (11.15 ± 0.85) × 10 5 μm 3 increased to 13.91 ± 1.4 μm, (15.86 ± 0.93) × 10 5 μm 3, and the microvessel density increased from 0 .0 2 2 ± 0. 0 2μm2 / μm2 increased to 0. 6 1 ± 0. 0 3μm2 / μm2, with the expansion of the prolongation of the observation value after the appearance of a transient decline again showed the same change. The flow rate increased after expansion and maintained an average level of 10 12 .45 μm / s on average. Conclusion The expansion of the skin about 3 to 4 weeks to complete a micro-vascular hyperplasia structure evolution cycle, to achieve a maximum diameter, flow and density.