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目的观察还原型谷胱甘肽(GSH)对链脲佐菌素(STZ)诱导的糖尿病心肌病(DCM)大鼠心肌线粒体功能的影响。方法将SD大鼠随机分为正常对照组(C组)和糖尿病组,糖尿病组腹腔注射STZ建立糖尿病大鼠模型。将建模成功的大鼠随机分为糖尿病心肌病模型组(DCM组)和GSH干预组(DCM+GSH组),DCM+GSH组腹腔注射GSH,C组和DCM组腹腔注射生理盐水。8周后,检测各组大鼠血清及心肌组织匀浆中的抗氧化指标、Ca2+诱导的线粒体肿胀度的变化及心肌线粒体的膜电位。结果DCM+GSH组大鼠心肌组织匀浆中超氧化物歧化酶活性较DCM组显著升高,血清中SOD活性较DCM组有升高趋势,但差异无统计学意义;DCM+GSH组大鼠血清中丙二醛(MDA)含量较DCM组显著下降,心肌组织匀浆中MDA含量较DCM组有下降趋势,但差异无统计学意义;DCM+GSH组大鼠心肌线粒体对高钙诱导的肿胀的敏感性较DCM组有所恢复,但仍未恢复到正常水平;DCM+GSH组大鼠心肌线粒体膜电位较DCM组显著上升。结论GSH对STZ诱导的DCM大鼠的心脏有一定的保护作用,其可能的机制为增强心肌抗氧化能力及改善心肌组织中线粒体的功能。
Objective To observe the effect of reduced glutathione (GSH) on myocardial mitochondrial function in streptozotocin (STZ) -induced diabetic cardiomyopathy (DCM). Methods SD rats were randomly divided into normal control group (C group) and diabetes mellitus group. Diabetic rats were intraperitoneally injected with STZ to establish a diabetic rat model. The rats with successful modeling were randomly divided into diabetic cardiomyopathy model group (DCM group) and GSH intervention group (DCM + GSH group). Rats in DCM + GSH group were intraperitoneally injected with GSH, and C and DCM groups were intraperitoneally injected with normal saline. After 8 weeks, the anti-oxidant indexes in serum and myocardial homogenate, Ca2 + -induced mitochondrial swelling and myocardial mitochondrial membrane potential were detected. Results The activity of superoxide dismutase in myocardial homogenate of DCM + GSH group was significantly higher than that of DCM group, while the activity of SOD in serum was higher than that of DCM group, but the difference was not statistically significant. MDA content decreased significantly in DCM group compared with DCM group, while the content of MDA in myocardial homogenate decreased compared with DCM group, but the difference was not statistically significant. The myocardial mitochondria in DCM + GSH group had no effect on calcium-induced swelling Compared with DCM group, the sensitivity of DCM + GSH group was recovered, but not returned to normal level. The mitochondrial membrane potential of DCM + GSH group increased significantly compared with DCM group. Conclusions GSH can protect the heart of DCM rats induced by STZ. The possible mechanism is that GSH can enhance myocardial antioxidant capacity and improve mitochondrial function in myocardial tissue.