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目的:了解食管癌发生过程中的炎症改变.方法:采用100?g/mL的4NQO通过饮水作用于C57BL/6小鼠,分别通过食管拉网脱落细胞法、碘染色法及病理组织学观察第12、16、20、24周等时间段的C57BL/6小鼠食管鳞状细胞癌建模病理的改变.结果:食管拉网脱落细胞法、碘染色法均未观察到小鼠早期食管病变,在实验第12周,纵向解剖食管,通过病理组织学观察到食管上皮不典型增生,第16、20、24周分别观察到原位癌、浸润性鳞癌的发生,在整个肿瘤的发生、发展过程中伴随炎症细胞浸润.结论:食管拉网脱落细胞法、碘染色法不适用于C57BL/6小鼠食管鳞癌建模形态学观察,只能通过病理组织学才能够在不同的时间段观察到食管癌的发生、发展及其炎症改变过程.
Objective: To investigate the inflammatory changes in the process of esophageal carcinogenesis.Methods: C57BL / 6 mice were treated with 100 μg / mL 4NQO by drinking water, and then were isolated by esophageal detachment, iodine staining and histopathological observation The changes of pathological changes in esophageal squamous cell carcinoma of C57BL / 6 mice at equal time intervals of 12, 16, 20, 24 weeks were observed.Results: No esophageal lesions were observed in esophageal exfoliated cells and iodine staining, In the twelfth week of experiment, the esophagus was longitudinally dissected, esophageal atypical hyperplasia was observed by histopathology, and the occurrence of in situ carcinoma and invasive squamous carcinoma were observed at the 16th, 20th and 24th weeks respectively. Accompanied by inflammatory cell infiltration.Conclusion: Esophageal detachment cells method and iodine staining method are not suitable for modeling the morphology of esophageal squamous cell carcinoma in C57BL / 6 mice, which can only be observed by histopathology at different time points To the occurrence and development of esophageal cancer and its inflammatory process.