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移动抑制试验(Migration inhibi-tion test,MIT)是检测机体特异性细胞免疫功能的常用方法之一。它已应用于测定传染、肿瘤、自身免疫病等病人机体细胞免疫状态的特异性诊断和有关发病机理的探讨等方面。 MIT一般是用豚鼠腹腔渗出细胞作为指示细胞。MIT同其它生物学试验一样,受很多因素影响。影响豚鼠腹腔渗出细胞移动的因素对MIT的结果有密切关系。为了使获得的结果可靠而且重复性强,本文对豚鼠腹腔渗出细胞移动影响因素加以探讨。材料和方法一、器材 1.毛细管:日本广株式会社制,内径1.45mm,长75mm,一端火焰封闭。 2.培养用小室:内径200mm,高
Migration inhibition test (MIT) is one of the commonly used methods to detect body-specific cellular immune function. It has been used to determine the specific diagnosis of cellular immune status in patients with infectious diseases, tumors, autoimmune diseases and other aspects of the pathogenesis. MIT generally uses guinea pig peritoneal exudate cells as indicator cells. Like other biological experiments, MIT is affected by many factors. The factors affecting the migration of peritoneal exudate cells in guinea pigs are closely related to the results of MIT. In order to make the obtained results reliable and reproducible, this article discusses the factors affecting the movement of peritoneal exudate cells in guinea pigs. Materials and Methods 1. Equipment 1. Capillary: manufactured by Nippon Kogyo Co., Ltd., inner diameter 1.45mm, length 75mm, one end flame closed. 2. Cultivation chamber: 200mm internal diameter, high