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目的通过研究茶多酚对精索静脉曲张大鼠睾丸组织氧化应激和细胞凋亡的影响,探讨茶多酚是否对精索静脉曲张大鼠生精功能具有保护作用,为临床上应用茶多酚治疗精索静脉曲张提供理论依据和实验基础。方法 32只雄性青春期Wistar大鼠随机分为4组,每组8只。其中第Ⅰ组为假手术组(仅游离左肾静脉,不结扎);第Ⅱ组为模型组(建立精索静脉曲张模型);第Ⅲ、Ⅳ组为茶多酚干预组(先建立精索静脉曲张模型,然后分别给予不同浓度的茶多酚干预)。造模4周后,第Ⅰ组及第Ⅱ组均给予生理盐水1mL/100g,第Ⅲ组给予茶多酚10mg/kg[即:(1mL盐水+1mg茶多酚)/100g],第Ⅳ组给予茶多酚40 mg/kg[即:(1mL盐水+4mg茶多酚)/100g];1次/d,灌胃,持续4周。4周后4组大鼠均处死取左侧睾丸组织,分别测定超氧化物歧化酶(SOD)活力、丙二醛(malondialdehyde,MDA)含量及检测凋亡指数(apoptosis index,AI)。结果第Ⅰ组SOD活力最高,第Ⅱ组低于第Ⅲ组和第Ⅳ组,差异有统计学意义(P<0.05);第Ⅰ组MDA含量最低,第Ⅲ、Ⅳ组低于第Ⅱ组,差异有统计学意义(P<0.05);第Ⅰ组AI最低,第Ⅲ、Ⅳ组AI低于第Ⅱ组,差异有统计学意义(P<0.05)。结论茶多酚能显著降低精索静脉曲张大鼠睾丸组织的氧化应激和细胞凋亡,间接改善精索静脉曲张大鼠睾丸的生精功能。
Objective To study the effects of tea polyphenols on oxidative stress and apoptosis of testis in rats with varicocele and to explore whether tea polyphenols can protect spermatogenic function of varicocele rats. Phenolic treatment of varicocele provides the theoretical basis and experimental basis. Methods Thirty-two male adolescent Wistar rats were randomly divided into 4 groups with 8 rats in each group. The first group was sham operation group (free left renal vein only, no ligation); the second group was the model group (the establishment of varicocele model); the third and the fourth group was the tea polyphenols intervention group Varicose veins model, and then were given different concentrations of tea polyphenols intervention). After 4 weeks of modeling, group I and group II were given physiological saline 1mL / 100g, group III was given tea polyphenols 10mg / kg [ie: (1mL saline + 1mg tea polyphenol) / 100g], group IV Give polyphenols 40 mg / kg [ie: (1 mL saline + 4 mg polyphenols) per 100 g] once daily for 4 weeks. After 4 weeks, the left testis was sacrificed in all 4 groups. The activity of superoxide dismutase (SOD), the content of malondialdehyde (MDA) and the apoptosis index (AI) were measured. Results The SOD activity in group Ⅰ was the highest, that in group Ⅱ was lower than that in group Ⅲ and group Ⅳ (P <0.05); the content of MDA in group Ⅰ was the lowest, the levels in group Ⅲ and Ⅳ were lower than those in group Ⅱ, The difference was statistically significant (P <0.05). The AI in group I was the lowest, and the AI in group III and IV was lower than that in group II (P <0.05). Conclusion Tea polyphenols can significantly reduce the oxidative stress and apoptosis of testicular tissue of rats with varicocele and indirectly improve the testicular spermatogenic function in varicocele rats.