目的改进月桂氮酮中有关物质的测定方法。方法采用气相色谱法,以甲醇为溶剂、Agilent DB-1毛细管柱(30m×0.53 mm,3.0μm)、程序升温、氢火焰离子化检测器进行测定。结果所用色谱条件可使月桂氮酮及其强降解杂质得到有效分离,专属性、重复性良好;杂质己内酰胺的检出限为0.13μg·m L-1,线性方程为:Y=2.3511×103X-0.2742,10.78~269.5μg·m L-1己内酰胺与峰面积的线性关系良好(r=0.9984)。结论改进后的方法操作简便,准确可靠,可有效地检测月桂氮酮中的有关物质。 Objective To improve the method for the determination of related substances in azadirachtin. Methods Gas chromatography was used to determine the temperature and hydrogen flame ionization detector using methanol as the solvent and Agilent DB-1 capillary column (30m × 0.53 mm, 3.0μm). Results The chromatographic conditions were used to effectively separate the azinon ketone and its degradation products. The detection limit of impurity caprolactam was 0.13μg · m L-1. The linear equation was Y = 2.3511 × 103X -0.2742,10.78 ~ 269.5μg · m L-1 caprolactam with a good linear relationship (r = 0.9984). Conclusion The improved method is simple, accurate and reliable, which can effectively detect related substances in azinon.