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目的:探讨Ⅰ相代谢酶细胞色素P450 2E1(CYP2E1)基因RsaⅠ和PstⅠ位点多态性及吸烟、饮酒习惯与前列腺癌(PCa)发病风险的关系,并探讨基因与生活习惯在PCa发病中的联合作用。方法:采用PCR-RFLP技术检测109例原发性PCa患者及202例年龄匹配的男性非肿瘤患者外周血CYP2E1基因RsaⅠ和PstⅠ多态位点的基因型。结果:深吸烟(OR=2.29,95%CI:1.28～4.09)、重度吸烟史(OR=1.81,95%CI:1.02～3.22)等生活习惯为PCa易感因素。CYP2E1 C1/C1基因型与PCa易感性有显著相关(OR=1.71,95%CI:1.04～2.82),且与饮酒的联合作用明显与PCa易感性相关(OR=2.21,95%CI:1.06～4.59)。重度吸烟人群中,携带CYP2E1易感基因型(C1/C1)与不吸烟且携带非易感基因型(C1/C2或C2/C2)个体相比PCa的发病风险显著增高(OR=2.80,95%CI:1.20～6.56)。结论:携带CYP2E1易感基因型(C1/C1)并有烟酒嗜好者PCa的发病风险显著增高,且与烟草的暴露呈显著剂量反应关系。
Objective: To investigate the polymorphism of the Rsa I and Pst I loci of cytochrome P450 2E1 (CYP2E1) gene and the risk of smoking, drinking and prostate cancer (PCa) in phase Ⅰ metabolic enzymes and to explore the role of genes and lifestyle in the pathogenesis of PCa Joint action. Methods: PCR-RFLP was used to detect the genotypes of CYP2E1 Rsa I and Pst I polymorphisms in peripheral blood of 109 patients with primary PCa and 202 age-matched male non-tumor patients. Results: The lifestyle habits of deep smoking (OR = 2.29, 95% CI: 1.28-4.09) and heavy smoking history (OR = 1.81,95% CI: 1.02-3.22) were the predisposing factors for PCa. The genotype of CYP2E1 C1 / C1 was significantly associated with susceptibility to PCa (OR = 1.71, 95% CI: 1.04-2.82), and its association with alcohol consumption was significantly associated with PCa susceptibility (OR = 2.21, 95% CI: 4.59). The risk of developing PCa was significantly higher in the smokers than in non-smokers with non-smokers and nonsmokers (C1 / C2 or C2 / C2) (OR = 2.80, 95 % CI: 1.20 ~ 6.56). CONCLUSIONS: The risk of PCa carrying CYP2E1 susceptibility genotype (C1 / C1) and alcohol addicts is significantly increased, and there is a significant dose-response relationship with tobacco exposure.