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目的:对2011年西宁市腹泻病毒进行流行病学调查,对青海省腹泻病毒流行株和流行趋势进行统计分析,为腹泻病毒引起的腹泻提供实验室资料,更好地控制病毒性腹泻的流行。方法:用巢式PCR和多重PCR方法对西宁地区488例婴幼儿腹泻标本进行腹泻病毒检测。结果:西宁地区腹泻儿童中腹泻病毒的检出率分别为:轮状病毒A组阳性33例(6.76%),其中G9型15例、G3型13例、P6型1例、P8型2例、P9型2例;轮状病毒B/C组阳性3例(0.61%);杯状病毒阳性23例(4.71%),其中扎如病毒17例、诺如病毒GⅡ型6例;星状病毒阳性7例(1.43%)。结论:轮状病毒A组和杯状病毒是西宁地区婴幼儿腹泻的主要病原体之一。
OBJECTIVE: To carry out a epidemiological investigation of the diarrhea virus in Xining City in 2011, and to analyze the prevalence and epidemiology of the diarrhea virus in Qinghai Province and to provide laboratory data for diarrhea caused by diarrhea virus to better control the prevalence of viral diarrhea. Methods: Diarrhea virus was detected in 488 cases of infantile diarrhea in Xining area by nested PCR and multiplex PCR. Results: The positive rates of diarrhea virus in diarrhea children in Xining were 33 cases (6.76%) of rotavirus A group, 15 cases of G9, 13 cases of G3, 1 case of P6 and 2 cases of P8, P9 type 2 cases; rotavirus B / C group was positive in 3 cases (0.61%); calicivirus positive in 23 cases (4.71%), including Zarubin 17 cases, Norovirus G Ⅱ 6 cases; astrovirus positive 7 cases (1.43%). Conclusion: Rotavirus group A and calicivirus are one of the major pathogens of infant diarrhea in Xining area.