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目的 :对幽门螺杆菌L型的生物学特性与致病性进行研究。方法 :在体外用羧苄西林诱导幽门螺杆菌形成L型以研究其生物学特性。分别取幽门螺杆菌原菌与L型感染实验小鼠以测定其生物学特牲与致病性。取 136份胃癌与 30份慢性胃炎患者标本进行组织学检查和细菌分离。结果 :体外诱导的L型与原菌在特性上差别很大 ,但仍能以免疫酶染色与PCR法识别。实验动物感染 ,在体内的L型呈圆球体 ,大多在胃与十二指肠黏膜引起间质性炎症 ,一只脾脏并见有恶性病变。临床胃癌标本检查见L型亦呈圆球体状 ,77.3%患者幽门螺杆菌抗原阳性。慢性胃炎胃液分离 ,除见有幽门螺杆菌外 ,并有多种细菌和L型存在。结论 :幽门螺杆菌L型形态多变 ,其中以圆球体多见于病变部位 ,且导致间质性和肿瘤的形成。但与慢性胃炎的关系尚有待进一步研究。
Objective: To study the biological characteristics and pathogenicity of Helicobacter pylori L-form. Methods: In vitro, carbenicillin was used to induce H. pylori to form L-form to study its biological characteristics. Helicobacter pylori isolates and L-infected mice were used to determine their biological characteristics and pathogenicity. 136 specimens of gastric cancer and 30 specimens of chronic gastritis were collected for histological examination and bacterial isolation. Results: The characteristics of L - type and in vitro - induced L - type strains were quite different, but they could still be identified by immunoenzyme staining and PCR. Experimental animal infection, in the body of the L-shaped ball, mostly in the stomach and duodenal mucosa caused by interstitial inflammation, a spleen and see malignant lesions. Clinical gastric cancer specimens also found that L-shaped was also spherical, 77.3% of patients with positive H. pylori antigen. Chronic gastritis gastric juice separation, in addition to see Helicobacter pylori, and a variety of bacteria and L-type existence. Conclusion: The Helicobacter pylori L - shaped morphological changes, which are more common in the lesion of the sphere, and lead to the formation of interstitial and tumor. However, the relationship with chronic gastritis remains to be further studied.