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目的:探讨大黄素对大鼠肝移植急性排斥反应的作用机制。方法:以近交系大鼠建立原位肝移植模型,分为3组,每组各16对:BN→BN同基因对照组,Lewis→BN急性排斥组,Lewis→BN大黄素干预组。各组随机选取6只受体大鼠,于术后1,3,5,7 d的取尾静脉血样,ELISA法检测TNF-α,IL-10含量,并于第7日处死,检测病理组织学改变。其余每组各10只观察其生存状况和生存期。结果:大黄素干预组术后中位生存时间为25.6 d,较排斥组10.9 d明显延长(P<0.05);与排斥组相比,大黄素干预组肝脏组织病理排斥反应明显降低(P<0.01),外周血TNF-α的含量明显下降(P<0.05),IL-10上调,自术后3 d有统计学差异(P<0.05)。结论:大黄素能够有效抑制肝移植大鼠的急性排斥反应,其作用与降低外周血TNF-α的含量,上调IL-10水平有关。
Objective: To investigate the mechanism of emodin on acute rejection of liver transplantation in rats. Methods: Orthotopic liver transplantation models were established in inbred rats and divided into 3 groups with 16 pairs in each group: BN→BN isogenic control group, Lewis→BN acute rejection group, and Lewis→BN emodin intervention group. Six recipient rats were randomly selected from each group. The tail vein blood samples were taken at 1, 3, 5, and 7 days after operation. The levels of TNF-α and IL-10 were detected by ELISA. The rats were sacrificed on the 7th day to examine the pathological tissues. Learn to change. The remaining 10 animals in each group were observed for their survival and survival. Results: The median survival time of the emodin intervention group was 25.6 days, which was significantly longer than that of the rejection group (P<0.05). Compared with the rejection group, the liver histopathological rejection was significantly lower in the emodin intervention group (P<0.01). ), The content of TNF-α in peripheral blood was significantly decreased (P<0.05), IL-10 was up-regulated, and there was a statistical difference (P<0.05) from the 3rd day after operation. CONCLUSION: Emodin can effectively inhibit acute rejection in rats after liver transplantation. Its effect is related to the decrease of TNF-α content in peripheral blood and up-regulation of IL-10 level.