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AIM:To investigate alternative splicing in vascular endothelial growth factor A(VEGFA),amyloid beta precursor protein(APP),and Numb homolog(NUMB) in colorectal cancer(CRC).METHODS:Real-time quantitative reverse transcriptase polymerase chain reaction(q RT-PCR) and PCRrestriction fragment length polymorphism analyses were performed to detect the expression of VEGFA,APP,and NUMB mR NA in 20 CRC tissues and matched adjacent normal tissues,as well as their alternative splicing variants.RESULTS:q RT-PCR analysis revealed that the expression of APP,NUMB,and VEGFA 165 b m RNA were significantly downregulated,while VEGFA m RNA was upregulated,in CRC tissues(all P < 0.05).PCRrestriction fragment length polymorphism analysis revealed that the expression of VEGFA 165a/b in CRC tissues was significantly higher than in adjacent normal tissues(P < 0.05).Compared with adjacent normal tissues,the expression of NUMB-PRRS in CRC tissues was significantly decreased(P < 0.05),and the expression of NUMB-PRRL was increased(P < 0.05).CONCLUSION:Alternative splicing of VEGFA,APP,and NUMB may regulate the development of CRC,and represent new targets for its diagnosis,prognosis,and treatment.
AIM: To investigate alternative splicing in vascular endothelial growth factor A (VEGFA), amyloid beta precursor protein (APP), and Numb homolog (NUMB) in colorectal cancer (CRC). METHODS: Real-time quantitative reverse transcriptase polymerase chain reaction RT-PCR) and PCR restriction fragment length polymorphism analyzes were performed to detect the expression of VEGFA, APP, and NUMB mR NA in 20 CRC tissues and matched adjacent normal tissues, as well as their alternative splicing variants. RESULTS: q RT-PCR analysis revealed that the expression of APP, NUMB, and VEGFA 165 bm RNA were significantly downregulated, while VEGFA m RNA was upregulated in CRC tissues (all P <0.05). PCR Restriction fragment length polymorphism analysis revealed that the expression of VEGFA 165a / b in The CRC tissues were significantly higher than in adjacent normal tissues (P <0.05) .Compared with adjacent normal tissues, the expression of NUMB-PRRS in CRC tissues was significantly decreased (P <0.05), and the expression of NUMB-PR RL was increased (P <0.05). CONCLUSION: Alternative splicing of VEGFA, APP, and NUMB may regulate the development of CRC, and represent new targets for its diagnosis, prognosis, and treatment.