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目的 研究THANK在肝癌免疫治疗中的作用,在体内外实验研究中证实THANK对肝癌细胞系SMMU-7721的免疫调节作用。方法 将THANK全长cDNA转染入人肝癌细胞株SMMU-7721中,经克隆筛选,获取高表达THANK的SMMU-7721肝癌细胞株(THANK-7721细胞),体外分析THANK对7721细胞生物学特性的影响,并在裸鼠体内检测其成瘤性的变化。结果 THANK在7721细胞中的表达可抑制7721细胞的生长,使肿瘤细胞的生长停滞于S期,且可在体外诱导强烈的CTL反应,人PBMC对THANK-7721细胞的杀伤活性明显增强。而在裸鼠体内的实验表明,THANK在7721细胞中的表达不仅可降低其成瘤性,且在裸鼠体内接种THANK-7721细胞可引起机体一定的免疫反应,抵抗再次接种的亲代7721细胞的生长。 结论 THANK基因可显著地调节人肝癌细胞SMMU-7721的免疫原性,有希望用于肝癌的免疫治疗。
Objective To study the role of THANK in hepatocellular carcinoma (HCC) immunotherapy and to confirm the immunoregulatory effect of THANK on hepatocellular carcinoma cell line SMMU-7721 in vitro and in vivo. Methods THANK full-length cDNA was transfected into human hepatocellular carcinoma cell line SMMU-7721 and cloned to obtain the THANK-7721 hepatoma cell line THANK-7721 which was highly expressed by THANK. The biological characteristics of THANK 7721 cells were analyzed in vitro Effect, and detect the change of tumorigenicity in nude mice. Results The expression of THANK in 7721 cells could inhibit the growth of 7721 cells, arrest the growth of tumor cells in S phase and induce strong CTL reaction in vitro. The cytotoxic activity of THANK-7721 cells was significantly enhanced by THANK. In vivo experiments in nude mice showed that THANK expression in 7721 cells not only reduced its tumorigenicity, but also in vivo nude mice inoculated with THANK-7721 cells can cause a certain immune response against re-vaccination of 7721 cells Grow. Conclusion THANK gene can significantly regulate the immunogenicity of SMMU-7721 human hepatocellular carcinoma cells, and is promising for the immunotherapy of hepatocellular carcinoma.