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本文报道应用大鼠实验性局灶脑梗塞的体积定量测算法,研究小檗胺的抗脑缺血作用。在Wistar大鼠电凝左侧大脑中动脉制造局灶脑梗塞。实验组在手术前和手术完毕时腹腔注射盐酸小檗胺4mg,第2日注射2次。实验组同法注射生理盐水。第3日取脑,灌注固定。作连续切片,每只大鼠取8～12张切片。计算出每只鼠的前脑体积、脑梗塞体积和脑梗塞占前脑体积百分率。实验组与对照组的平均脑梗塞占前脑体积百分率分别为10.0％和10.2％。小檗胺不易透过血脑屏障,无脑保护作用。实验结果表明,脑梗塞体积定量检测法应用于普通大鼠筛选抗脑缺血药是有效的。
This article reports the quantitative determination of focal cerebral infarction in rats using quantitative method to study the anti-cerebral ischemia berbamine effect. Focal cerebral infarction was created by electrocoagulation of the left middle cerebral artery in Wistar rats. In the experimental group, 4mg berbamine hydrochloride was injected intraperitoneally before surgery and at the end of the operation, and injected twice on the second day. The same experimental group injected with saline. On the third day, take the brain, perfusion fixed. For serial sections, each rat to take 8 to 12 sections. The forebrain volume, infarct volume, and brain infarct volume per forebrain were calculated for each mouse. The mean percentage of cerebral infarction to forebrain in the experimental and control groups was 10.0% and 10.2%, respectively. Berbamine difficult to penetrate the blood-brain barrier, no brain protection. The experimental results show that the quantitative detection of cerebral infarction volume applied to the general rat screening of anti-cerebral ischemia is effective.