背景:用于骺板部分早闭的实验动物模型多为周围型骺板部分早闭。目的:在周围型骺板部分早闭模型的基础上,改进建立股骨远端周围型骺板部分早闭动物模型。设计、时间及地点:自身对照设计实验,于2007-03/10在解放军总医院实验动物中心完成。材料:健康普通级新西兰幼兔20只,兔龄4~6周,体质量1.0~1.5kg。方法:将20只新西兰白兔取膝外侧直切口,长约2cm,切开皮肤、皮下组织,并于外侧髌旁切开膝关节囊后显露股骨远端外髁,此时股骨远端的骺板线清晰可见。将右侧股骨外髁的骺板挖两个相邻的孔,深度约3.0~4.0mm,以去除部分正常骺板,面积为股骨远端骺板总面积的33%~44%。左侧仅切开至骨膜作为自身对照。主要观察指标:术后X射线片测量同时大体观察双侧股骨的长度、股骨远端外翻角度及股骨的畸形情况;组织学检查双侧股骨远端的骨桥形成情况。结果:1只实验兔术后31d因腹泻死亡,19只术后生长良好,进入结果分析。①术后4个月,X射线观察显示左侧股骨平均长度>右侧,外翻角度<右侧,两组之间相比,差异显著(P<0.01)。股骨远端左侧平均生长2.27cm,占股骨生长的57.6%,右侧平均生长0.45cm,占股骨生长的21.4%。②术后4个月,对照组股骨形态正常,两髁及髌骨关节面光滑完整。实验侧股骨明显短缩,远端出现严重的外翻畸形。③术后5周,对照组骺板各层细胞排列整齐。实验组骨缺损腔内可见大量纤细的骨小梁,周围剩余骺板细胞层数减少。术后10周,对照组骺板已基本闭合;实验组骨小梁变得更为粗大。结论:本方法建立的模型操作简单,成功率高,应用灵活,造成的骨桥面积大小可以控制,适用骺板部分早闭疾病动物模型的要求,对治疗过程中充填物的选择亦有一定意义。 BACKGROUND: Experimental animal models used for partial early closure of the epiphyseal plate are mostly partially closed early in the peripheral epiphyseal plate. OBJECTIVE: To improve the establishment of partial early-closure animal models of the peripheral epiphyseal plate on the basis of the partial early-closure model of the peripheral epiphyseal plate. DESIGN, TIME AND SETTING: The self-designed control experiment was performed at the Experimental Animal Center of Chinese PLA General Hospital on March 10, 2007. MATERIALS: Twenty healthy New Zealand rabbits were aged 4 to 6 weeks and their body weight was 1.0 ~ 1.5kg. METHODS: Twenty New Zealand white rabbits were cut out of the knee lateral to a length of 2 cm to cut the skin and subcutaneous tissue. The lateral femoral condyle was exposed after the knee joint capsule was excised on the lateral patellar side. At this time, the epiphysis of the distal femur Plate line is clearly visible. The epiphyseal right femoral condyle digging two adjacent holes, a depth of about 3.0 ~ 4.0mm, in order to remove part of the normal epiphyseal plate, the area for the distal femoral epiphyseal plate total area of ​​33% to 44%. Only the left side of the periosteum was cut as self-control. MAIN OUTCOME MEASURES: The length of bilateral femur, valgus distal femoral valgus and femoral deformity were observed at the same time after surgery. Histopathological examination of the formation of the bridge of the distal femur. Results: One experimental rabbit died of diarrhea on the 31st day after operation, and 19 patients developed well after operation. Results were analyzed. ① At 4 months after operation, X-ray showed that the left femur average length> right side and valgus angle was