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目的:比较肝癌大鼠在体应用干扰素、卡介苗或二者联用后.几种单核巨噬细胞体外抗肝癌细胞作用的变化.方法:以二乙基亚硝胺诱发大鼠肝癌,实验组同时给予干扰素、卡介苗或二者联用.于第8、12、16周分离肝枯否细胞、腹腔巨噬细胞、肺巨噬细胞、脾巨噬细胞和血单核细胞,分别与人肝癌细胞联合培养.用H-TdR掺入法测定5种细胞抑肝癌细胞增殖作用.结果:实验组肝病理变化明显轻于对照组.以干扰素与卡介苗联用组病变最轻.实验组5种细胞体外抗肝癌细胞作用明显增强,卡介苗的作用强于干扰素.尤以两者联用组效果最佳,使效应细胞作用增强1～5倍.结论:研究为肝癌治疗合理选用免疫制剂提供了实验依据.
OBJECTIVE: To compare the effects of several monocyte-macrophages on the anti-hepatocarcinoma cells in vitro after the application of interferon, BCG, or a combination of both in hepatoma rats. METHODS: Rat liver cancer induced by diethylnitrosamine was tested. The rats were given either interferon, BCG, or a combination of both. Liver Kupffer cells, peritoneal macrophages, pulmonary macrophages, splenic macrophages, and blood mononuclear cells were isolated on the 8th, 12th, and 16th weeks respectively. Hepatoma cells were co-cultured. The proliferation of 5 hepatocarcinoma cells was measured by H-TdR incorporation. Results: The hepatic pathological changes in the experimental group were significantly lighter than those in the control group. The combination of interferon and BCG was the least severe. Experimental group 5 The anti-hepatocarcinoma cells in vitro significantly enhanced the role of the seed cells. The effect of BCG was stronger than that of the interferon. Especially, the effect of the two combined groups was the best, and the effector cells were enhanced by 1 to 5 times. Conclusion: The study provides a rational selection of immune preparations for the treatment of liver cancer. The experimental basis.