论文部分内容阅读
Objective: To observe expression of SIRT3 in normal liver tissue, cirrhotic tissue and hepatocellular carcinoma (HCC) tissues, and to explore the significance of SIRT3 in primary HCC . Methods: SIRT3 expression was detected in 10 normal cases, 30 cases with, 30 HCC cases by immunohistochemical and Western-blotting method. Results: Immunohistochemical assay showed that the SIRT3 positive expression rates were 100.0% (10/10), 96.7% (29/30) and 60.0% (18/30), respectively in normal group, paracancer group and HCC group. And the SIRT3 expression in HCC group was significantly lower than in normal group and paracancer group ( P<0.05). Western-blotting showed the SIRT3 expression in cancer tissue was 0.29暲0.07, significantly lower than that in paracancer group and normal group ( P<0.05). SIRT3 expression was related to the differentiation degree and portal vein tumor thrombus ( P<0.05). Conclusions: Abnormal expression of SIRT3 is closely related to the biological behavior of primary HCC.
Objective: To observe expression of SIRT3 in normal liver tissue, cirrhotic tissue and hepatocellular carcinoma (HCC) tissues, and to explore the significance of SIRT3 in primary HCC. Methods: SIRT3 expression was detected in 10 normal cases, 30 cases with, 30 HCC cases by immunohistochemical and Western-blotting method. Results: Immunohistochemical assay showed that the SIRT3 positive expression rates were 100.0% (10/10), 96.7% (29/30) and 60.0% (18/30) respectively in normal group, paracancer group and HCC group. And the SIRT3 expression in HCC group was significantly lower than in normal group and paracancer group (P <0.05). Western-blotting showed the SIRT3 expression in cancer tissue was 0.29 暲 0.07, significantly lower than that in paracancer group and normal group (P <0.05). SIRT3 expression was related to the differentiation degree and portal vein tumor thrombus (P <0.05). Conclusions: Abnormal expression of SIRT3 is closely related to the biological behavior of primary HCC.