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研究了在铂丝电极上电聚合一层带正电的2氨基吡啶膜,然后再利用层层自组装技术固定纳米金、电子媒介体硫堇及辣根过氧化氢酶,从而制备了由辣根过氧化氢酶/纳米金/硫堇/纳米金/聚2氨基吡啶膜修饰的酶生物传感器.实验中探讨了聚合层数、温度、pH值等对电极响应的影响.结果表明该传感器在H2O2浓度6·0×10-7~1·3×10-3mol/L范围内呈线性响应,检出限为2·1×10-7mol/L.此外,该传感器具有好的稳定性和选择性,能有效排除抗坏血酸、柠檬酸、葡萄糖等常见物质的干扰.
The electroplating of a 2-aminopyridine membrane with a positive charge on a platinum wire electrode was studied, and then nano gold, electron mediator thionine and horseradish peroxidase were immobilized by a layer-by-layer self-assembly technique, Root catalase / nano gold / thionine / nano gold / poly 2 aminopyridine membrane enzyme biosensor modified.In this experiment, the influence of the polymerization layer number, temperature, pH on the electrode response was investigated.The results show that the sensor H2O2 concentration of 6 · 0 × 10-7 ~ 1 · 3 × 10-3mol / L range of linear response with a detection limit of 2 × 10-7mol / L. In addition, the sensor has good stability and selection Sexual, can effectively exclude ascorbic acid, citric acid, glucose and other common substances interference.