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目的:探讨胃癌患者血管内皮生长因子C(vascular endothelial growth factor-C,VEGF-C及血管内皮生长因子受体-3(vascular endothelial growth factor receptor-3,VEGFR-3)在胃癌组织中的表达,从而确定胃癌预后的分子标志物。方法:搜集整理临床资料,采用Real-time PCR及ELISA法检测43例胃癌组织VEGF-C和VEGFR-3的表达。结果:43例胃癌组织中均有不同程度的VEGF-C和VEGFR-3的表达,Real-time PCR结果显示胃癌组织淋巴结转移组和非转移组VEGF-C和VEGFR-3的表达分别为0.07±0.01和0.12±0.01,0.03±0.01和0.06±0.02,与正常对照组相比,差异有显著性(p<0.05)。ELISA检测显示,与正常胃组织中VEGF-C和VEGFR-3的蛋白表达相比,胃癌无淋巴结转移组及胃癌并发淋巴结转移组中VEGF-C和VEGFR-3均明显增加。结论:VEGF-C和VEGFR-3的表达与胃癌淋巴结转移密切相关,提示胃癌标本VEGF-C和VEGFR-3的检测可作为胃癌预后的分子标志物。
Objective: To investigate the expression of vascular endothelial growth factor-C (VEGF-C) and vascular endothelial growth factor receptor-3 (VEGFR-3) in gastric cancer, So as to determine the prognosis of gastric cancer.Methods: The clinical data were collected and the expressions of VEGF-C and VEGFR-3 in 43 cases of gastric cancer were detected by Real-time PCR and ELISA.Results: All 43 cases of gastric cancer had different degrees Real-time PCR results showed that the expressions of VEGF-C and VEGFR-3 in lymph node metastasis group and non-metastasis group were 0.07 ± 0.01 and 0.12 ± 0.01, 0.03 ± 0.01 and 0.06 ± 0.02, compared with the normal control group, the difference was significant (p <0.05) .ELISA detection showed that compared with the normal gastric tissue VEGF-C and VEGFR-3 protein expression, gastric cancer without lymph node metastasis and gastric cancer complicated VEGF-C and VEGFR-3 in lymph node metastasis group were significantly increased.Conclusion: The expression of VEGF-C and VEGFR-3 is closely related to the lymph node metastasis of gastric cancer, suggesting that the detection of VEGF-C and VEGFR-3 in gastric cancer specimens can be used as prognosis of gastric cancer Molecular markers.