为了协同表达MHCII类分子的两个链α和 β ,利用脑心肌炎病毒和脊髓灰质炎病毒的内核糖体进入位点 ,构建了含MHCII类分子α和 β链及选择标记新霉素磷酸转移酶基因的三顺反子逆转录病毒载体。经包装细胞包装成重组逆转录病毒后 ,感染NIH3T3、MM45T Li、COS7细胞 ,经PCR、RT PCR、Southern印迹、Northern印迹及流式细胞术在多水平上证实了α链和 β链的基因表达和MHCII类分子的正确组装。 In order to co-express the two chains of α and β chains of MHC class II molecules, the intranucleosomes of encephalomyocarditis virus and poliovirus were used to enter the site, and the α and β chains containing MHC class II molecules and the neomycin phosphotransferase Gene of the tricistronic retroviral vector. After packaged cells were packaged into recombinant retroviruses, NIH3T3, MM45T Li, and COS7 cells were infected and gene expression of alpha and beta chains was confirmed at various levels by PCR, RT PCR, Southern blot, Northern blot and flow cytometry And the correct assembly of MHC class II molecules.