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目的探讨维吾尔族和汉族妇女中宫颈炎和宫颈癌组织中HSP90蛋白的表达和HPV16型感染的情况。方法采用免疫组织化学SP法和聚合酶链反应(PCR)技术检测66例宫颈炎组织和92例宫颈癌组织中HSP90蛋白的表达和HPV16的感染情况。结果HSP90蛋白在维、汉宫颈癌组织中的阳性率高于维、汉宫颈炎组织(77.17%,56.06%)(P<0.05);HSP90蛋白的阳性率表达在维、汉宫颈癌组织间差异有统计学意义(P<0.05),并且汉族高于维吾尔族(83.33%,74.19%)。在HPV16型感染的宫颈癌组织中维吾尔族阳性率(58.06%)高于汉族(16.67%)(P<0.05)。HPV16型感染在宫颈癌组织中的阳性率高于宫颈炎组织(44.56%,12.12%)(P<0.05)。维、汉宫颈癌组织中HSP90的表达与HPV16型感染呈正相关性(r=0.361,P<0.05)。结论通过对HSP90蛋白和HPV16联合检测,可能作为检查宫颈癌的标志物,提高宫颈癌的检出率。
Objective To investigate the expression of HSP90 protein and HPV16 infection in cervicitis and cervical cancer in Uighur and Han women. Methods Immunohistochemical SP method and polymerase chain reaction (PCR) were used to detect the expression of HSP90 protein and HPV16 in 66 cases of cervicitis and 92 cases of cervical cancer. Results The positive rate of HSP90 protein in cervical cancer tissues was significantly higher in Victoria and Han (77.17%, 56.06%) than that in Victoria and Han (P <0.05). The positive rate of HSP90 protein expression was different between Victoria and Han cervical cancer tissues (P <0.05), and Han people were higher than Uighur people (83.33%, 74.19%). The positive rate of Uyghur in cervical cancer tissues infected with HPV16 (58.06%) was higher than that in Han (16.67%) (P <0.05). The positive rate of HPV16 infection in cervical cancer was higher than that in cervicitis (44.56%, 12.12%) (P <0.05). There was a positive correlation between the expression of HSP90 and HPV16 infection in cervical cancer tissues of Victoria and Han (r = 0.361, P <0.05). Conclusion The combined detection of HSP90 protein and HPV16 may be used as a marker of cervical cancer and improve the detection rate of cervical cancer.