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胰液或十二指肠液内乳铁蛋白(Lacto-ferrin简称LF)的测定对胰腺疾病鉴别诊断是一种有价值的试验。慢性胰腺炎病人胰液和十二指肠液内LF浓度增高,而在胰腺癌或急性胰腺炎则不增高。为胰腺疾病的实验诊断建立LF放射免疫测定方法的需要,我们进行了人乳铁蛋白的分离提纯和抗血清制备。现将结果报告如下。 材料和方法 一、LF的分离提纯:参照Johansson和Haupt从人初乳分离和结晶LF的方法,收集产后1～3天的人初乳1,500ml经3,000rpm 30分钟低温离心除去上层乳脂,取
Determination of pancreatic juice or duodenal fluid lactoferrin (Lacto-ferrin referred to as LF) is a valuable test for the differential diagnosis of pancreatic disease. Chronic pancreatitis in patients with pancreatic juice and duodenal fluid LF concentration increased, while in pancreatic cancer or acute pancreatitis is not increased. For the experimental diagnosis of pancreatic diseases need to establish a method of LF radioimmunoassay, we carried out the separation and purification of human lactoferrin and antiserum preparation. The results reported below. Materials and Methods I. Separation and Purification of LF: According to the method of separating and crystallizing LF from human colostrum by Johansson and Haupt, 1,500 ml of colostrums of 1 to 3 days postpartum were collected and centrifuged at 3,000 rpm for 30 minutes to remove the upper milk fat,