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OBJECTIVE To investigate the effect of sonodynamic action on biofilm-producing Enterococcus faecalis using hypocrellin B from a natural product Hypocrella bambuase as a sensitizer.METHODS The uptake of hypocrellin B in biofilm-producing Enterococcus faecalis was measured using the fluorescent analysis method.After the combined treatment of hypocrellin B and ultrasound,the growth of biofilm-producing Enterococcus faecalis was measured using the colony counting method,the membrane integrity was detected using a flow cytometric analysis with propidium iodide staining.Chromosomal DNA of bacteria was analysed using apulsed-field gel electrophoresis(PFGE).RESULTS The uptake of hypocrellin B in biofilm-producing Enterocuccus faecalis reached a high peak at the 20 min incubation.After the combined treatment of hypocrellin B,the growth of biofilm-producing Enterocuccus faecalis significantly descreased and membrane integrity was remarkably damaged.However,no remarkable change in bacterial DNA was found.CONCLUSION Sonodynamic action of hypocrellin B had significant killing activity on Enterocuccus faecalis.
OBJECTIVE To investigate the effect of sonodynamic action on biofilm-producing Enterococcus faecalis using hypocrellin B from a natural product Hypocrella bambuase as a sensitizer. METHODS The uptake of hypocrellin B in biofilm-producing Enterococcus faecalis was measured using the fluorescent analysis method. After the combined treatment of hypocrellin B and ultrasound, the growth of biofilm-producing Enterococcus faecalis was measured using the colony counting method, the membrane integrity was detected using a flow cytometric analysis with propidium iodide staining. Chromosomal DNA of bacteria was analyzed using apulsed-field gel electrophoresis (PFGE) .RESULTS The uptake of hypocrellin B in biofilm-producing Enterocuccus faecalis reached a high peak at the 20 min incubation. After the combined treatment of hypocrellin B, the growth of biofilm-producing Enterocuccus faecalis significantly descreased and membrane integrity was remarkably damaged .However, no remarkable change in bacterial DNA w as found. CONCLUSION Sonodynamic action of hypocrellin B had significant killing activity on Enterocuccus faecalis.