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面向 HPL C- DAD分析 ,提出一种复杂分析体系计算解析方法 ,用于中药等复杂物质体系中有效组分的含量测定 .该方法将基线作为一个独立组分参与交替最小二乘法迭代 ,以减少对迭代计算过程的干扰 ,从而无需获取纯物质光谱 ,只要简单估计各组分浓度分布作为初始迭代矢量 ,就能在含有严重未知杂质干扰的情况下 ,有效地解析出色谱重叠峰中各组分谱峰和相应的纯物质光谱 .使用仿真数据和参麦注射剂实测数据进行计算考核 ,结果表明 ,该法具有计算简单易行、自动化程度高等优点 .
In the face of HPL C-DAD analysis, a complex analytical system calculation and analysis method is proposed for the determination of effective components in complex substances system such as traditional Chinese medicine, etc. The method takes the baseline as an independent component involved in the alternative least squares iterative method to reduce Therefore, it is not necessary to obtain the spectra of pure substances, so long as the concentration distribution of each component is simply estimated as the initial iteration vector, the components of the overlapping peaks can be effectively resolved under the interference of serious unknown impurities Spectral peak and the corresponding pure substance spectrum.Using the simulation data and the measured data of Shenmai injection, the results show that this method has the advantages of simple and easy calculation and high degree of automation.