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实验样品取自健康青年死后4～6小时的肝脏,置二倍体的 pH7.6磷酸盐缓冲液(PBS),0℃下匀浆、离心。上清液通过DEAE-Cellulose 柱,用 pH7.6 PBS 及 NaCl
The experimental samples were taken from the liver of healthy young people aged 4 to 6 hours after their death. Diploid pH 7.6 phosphate buffered saline (PBS) was homogenized at 0 ℃ and centrifuged. The supernatant was passed through a DEAE-Cellulose column with pH 7.6 PBS and NaCl