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目的 探讨单次ig杂色曲霉素 (ST)对小鼠大脑细胞的影响。方法 采用形态学观察和流式细胞术定量检测方法 ,研究ST对BALB/c小鼠大脑神经细胞的影响。结果 病理形态学观察可见 ,ig小剂量ST(3μg·kg-1 )后 1 2h或大剂量ST(3mg·kg-1 )后6h即可见小鼠大脑皮质、丘脑、海马CA2区神经元出现胞核固缩深染、胞浆嗜酸性变、空泡变性等病理变化 ,且随剂量增大和作用时间延长 ,病变神经元逐渐增多 ;光镜下对海马CA2区病变神经元进行计数分析 ,结果表明ST处理组发生病理变化的神经元比例均高于相应对照组 ,且呈剂量和时间依赖性增高 ;流式细胞术定量检测结果表明 ,igST 3,30 ,30 0和30 0 0 μg·kg-1 1 2h后 ,小鼠脑细胞的凋亡率呈剂量依赖性增高 ;ig3mg·kg-1 的ST后 6～48h,随ST作用时间的延长 ,脑细胞凋亡率也明显增高。结论经口给予ST可导致小鼠大脑皮质、丘脑、海马CA2区神经元发生退行性病变 ,诱导并促进小鼠大脑细胞凋亡
Objective To investigate the effect of single ig steriglitazone (ST) on mouse brain cells. Methods Morphological observation and flow cytometry quantitative detection of ST in BALB / c mouse brain cells. Results Pathomorphology showed that neurons in CA2 area of cerebral cortex, thalamus and hippocampus appeared in 12 h after ST (3 μg · kg-1) or 6 h after ST (3 mg · kg-1) Nuclear shrinkage and deep dyeing, cytoplasm eosinophilic changes, vacuolar degeneration and other pathological changes, and with increasing doses and the role of prolonged time, increasing neurons; under the light of the hippocampal CA2 lesion neurons count analysis showed that The proportion of neurons in pathological changes in ST treated group was higher than that in control group, and the dose-dependent and time-dependent increase. The results of flow cytometry showed that igST 3, 30, 30 0 and 30 0 0 μg · kg- The apoptotic rate of mouse brain cells increased in a dose-dependent manner after 1 12 hours. At 6 ~ 48 hours after ST treated with 3 mg · kg-1 ST, the apoptosis rate of brain cells was also significantly increased with the prolongation of ST. Conclusion Oral administration of ST can induce neuronal degeneration in the cerebral cortex, thalamus and hippocampus CA2 of mice and induce and promote the apoptosis of mouse brain cells