论文部分内容阅读
目的测定布氏田鼠对抗凝血灭鼠剂溴敌隆的敏感性,为检验该鼠的抗药性提供参数。方法实验室单鼠笼养无选择摄食试验:雌雄分开,随机分组,按5个不同食毒期供给浓度为0.0005%的溴敌隆毒饵;用Bliss计算机程序,计算不同食毒期与对应死亡率之间的关系。结果布氏田鼠对溴敌隆的敏感性性别之间差异无统计学意义(Pb>0.05,PLFP50>0.05);雌雄数据合并计算,得毒力回归线y=3.35+4.81x,LFP50和LFP99及其95%置信限分别为2.20(1.88～2.58)d和6.71(4.48～10.05)d。结论按世界卫生组织推荐的以致死99%敏感靶标鼠种的食毒期(LFP99)取整天数作为抗药性检验标准,布氏田鼠摄食浓度为0.0005%溴敌隆毒饵10d存活即为抗性鼠。
Objective To determine the susceptibility of Brandt’s vole to anticoagulant rodenticide bromadiolone and to provide parameters for testing its resistance. Methods Single squirrel cage feeding experiment with no choice was conducted: male and female were randomly divided into groups and fed with bromadiolone at a concentration of 0.0005% at five different feeding stages. Bliss computer program was used to calculate the relative mortality The relationship between. Results The sensitivities of Brandt’s voles to bromadiolone were not statistically different (Pb> 0.05, PLFP50> 0.05). The male and female data were combined to calculate the virulence regression line y = 3.35 + 4.81x, LFP50 and LFP99 95% confidence limits were 2.20 (1.88 ~ 2.58) d and 6.71 (4.48 ~ 10.05) d, respectively. Conclusion According to World Health Organization recommended lethal 99% sensitive target mice take food poisoning period (LFP99) take the whole number of days as the resistance test, Brandt’s voles fed concentration of 0.0005% bromadiolone bait 10d survival is resistance mouse.