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应用蛋白ｄｏｔｂｌｏｔ技术检测了低氧内皮细胞条件培养液（ＨＥＣＣＭ）和常氧内皮细胞条件培养液（ＮＥＣＣＭ）内ＰＤＧＦ相对含量，并利用［３Ｈ］－ＴｄＲ掺入法和流式细胞术观察了ＨＥＣＣＭ和ＮＥＣＣＭ及加入特异ＰＤＧＦ抗体对肺动脉平滑肌细胞（ＰＡＳＭＣ）生长的影响。结果表明，ＨＥＣＣＭ中的ＰＤＧＦ含量明显高于ＮＥＣＣＭ；ＨＥＣＣＭ能明显增强ＰＡＳＭＣ内ＤＮＡ合成，促进ＰＡＳＭＣ从Ｇｏ／Ｇ１期进入Ｓ期；当预先加入ＰＤＧＦ－Ｂ链抗体时，则会明显地抑制ＨＥＣＣＭ对ＰＡＳＭＣ的ＤＮＡ合成，阻止ＰＡＳＭＣ从Ｇｏ／Ｇ１期进入Ｓ期。结果提示，低氧时ＰＡＳＭＣ增殖与肺动脉内皮细胞分泌释放ＰＤＧＦ增加有关
The relative content of PDGF in hypoxic endothelial cell conditioned medium (HECCM) and normoxic endothelial cell conditioned medium (NECCM) was detected by dot blot technique and HECCM was observed by [3H] -TdR incorporation and flow cytometry And NECCM and adding specific PDGF antibody on the growth of pulmonary artery smooth muscle cells (PASMC). The results showed that the content of PDGF in HECCM was significantly higher than that in NECCM. HECCM significantly enhanced DNA synthesis in PASMC and promoted PASMC from Go / G1 phase to S phase. When PDGF-B chain antibody was pre-added, HECCM significantly inhibited HECCM PASMC DNA synthesis prevents PASMC from Go / G1 into S phase. The results suggest that PASMC proliferation in hypoxia is related to the increase of PDGF release from pulmonary artery endothelial cells