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目的研究豚鼠近视眼视网膜Müller细胞原代培养的方法。方法用半透明眼罩遮盖法建立豚鼠的近视眼模型。采用酶消化法培养豚鼠近视眼视网膜Müller细胞,用倒置相差显微镜观察Müller细胞的生长状况,以GFAP、Vimentin免疫组化染色进行细胞鉴定。结果半透明眼罩遮盖豚鼠右眼2周后,遮盖眼眼轴延长,近视形成。原代培养的视网膜Müller细胞贴壁生长,胞体较大、扁平,GFAP、Vimentin免疫组化染色呈阳性。结论酶消化法是培养豚鼠近视眼视网膜Müller细胞的一种有效方法。
Objective To study the method of primary culture of retinal Müller cells in guinea pig myopic eyes. Methods The guinea pig model of myopia was established by translucent blindfold method. Retinal Müller cells were cultured by enzymatic digestion method. The growth of Müller cells was observed by inverted phase contrast microscope, and the cells were identified by GFAP and Vimentin immunohistochemical staining. Results The translucent blindfold covered the right eye of the guinea pig for 2 weeks, covering the axial extension of the eye and the formation of myopia. Primary cultured retinal Müller cells adherent growth, large cell body, flat, GFAP, Vimentin immunohistochemical staining was positive. Conclusion Enzymatic digestion is an effective method to culture myelinated Müller cells in guinea pigs.