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用NADPH脱氢酶组织化学方法观察了大鼠主盆神经节及阴茎勃起组织内一氧化氮合酶(NOS)阳性成分的分布。发现主盆神经节内分布有大量NOS阳性神经元,其中大部分密集于盆神经进入端,而膀胱端稀少。阴茎深动脉及其分支螺旋动脉周围,阴茎和尿道海绵体的平滑肌小梁内均分布有NOS阳性纤维。阴茎勃起组织内未见NOS阳性神经元。将荧光金注人阴茎海绵体后,在主盆神经节内发现有较多的荧光金标记细胞。结合NADPH反应,发现这些荧光金标记细胞多为NOS阳性。本文结果证实主盆神经节内存在NOS阳性神经元,部分NOS阳性神经元支配阴茎勃起组织的血管床,提示一氧化氮可能是参与调节阴茎勃起的一种重要神经活性物质。
NADPH dehydrogenase histochemical method was used to observe the distribution of nitric oxide synthase (NOS) positive components in rat ganglia and penile erectile tissues. Found in the main ganglion distribution of a large number of NOS positive neurons, most of them concentrated in the pelvic nerve into the side, while the splenomegaly scarce. NOS-positive fibers were distributed in the smooth muscle trabeculae of deep penile and its branch spiral arteries, penis and urethral sponge. Penis erectile tissue no NOS positive neurons. Fluorescent gold injected into the penis, found in the main ganglion cells have more fluorescent gold-labeled cells. In combination with the NADPH reaction, most of these fluorescent gold-labeled cells were found to be NOS-positive. Our results confirm the presence of NOS-positive neurons in the main ganglion and some NOS-positive neurons dominate the vascular bed of penile erection, suggesting that nitric oxide may be an important neuroactive substance involved in the regulation of penile erection.