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利用静电吸附作用将抗体羊抗人免疫球蛋白G(anti-HIgG)修饰在银电极表面,制备了无标记型的免疫电极,采用电化学方法对人免疫球蛋白G(HIgG)进行分析测定。在0.1mol/L K3PO4-K2HPO4(pH 7.2)缓冲液中,加入的HIgG与银电极表面的anti-HIgG发生免疫反应,使anti-HIgG的电荷减少产生脱附,裸露出银,使电极有效面积增大,银溶出电流发生改变。利用电流变化和HIgG浓度之间的关系,实现对HIgG的分析测定,电流的变化量与HIgG的含量在0~140ng/mL范围内成正比关系。该法具有灵敏度高、无须标记,使用方便等优点。
The anti-human immunoglobulin G (anti-HIgG) was modified on the surface of silver electrode by electrostatic adsorption, and the immunolabeld electrode was prepared. The immunoglobulin G (HIgG) was analyzed by electrochemical method. In 0.1mol / L K3PO4-K2HPO4 (pH 7.2) buffer, the addition of HIgG anti-HIgG silver electrode surface immune reaction, anti-HIgG reduce the charge to produce desorption, bare silver, the electrode effective area Increase, silver dissolution current change. The relationship between the change of current and the concentration of HIgG was used to analyze and measure HIgG. The change of current was proportional to the content of HIgG in the range of 0 ~ 140ng / mL. The method has the advantages of high sensitivity, no marking, easy to use and so on.