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AIM:To investigate the protective effect of bifidobacterium in endotoxin-induced intestinal injury in preweaning rats.METHODS:Preweaning rats were randomly divided into three groups(n =40 for each):a control group(groupC),a model group(group E) and a treatment group(group T).Both groups E and T were intraperitoneally injected with lipopolysaccharide(LPS) at a dose of 5 mg/kg(5 mg/L in normal saline),and group T was intragastrically administrated with bifidobacterium suspension(2.0 × 109 CFU/m L,0.5 m L each time,twice a day,until the end of the experiment) 7 d before LPS administration.Group C was intraperitoneally injected with normal saline.After intraperitoneal injection and intragastric administration,the rats were placed back to the initial cage to receive breast feeding.The rats were killed at 2,6,12,24 or 72 h,respectively,after endotoxin or physiological saline injection to collect serum and ileal tissue samples.Myeloperoxidase(MPO) contents in serum and ileum were detected at different times,and expression of ileal defensin-5 m RNA was evaluated by reverse transcription-polymerase chain reaction.RESULTS:Serum and ileal MPO contents in group E were significantly higher than those in group C(serum contents:107.50 ± 17.70 vs 157.14 ± 24.67,P < 0.05; ileal contents:1.03 ± 0.21 vs 1.57 ± 0.33,P < 0.05),which peaked at 12 h and 6 h,respectively.MPO contents in group T were significantly lower than those in group E(serum contents:114.38 ± 24.56 vs 145.25 ± 23.62,P < 0.05; ileal contents:1.25 ± 0.24 vs 1.57 ± 0.33,P < 0.05).The expression of defensin-5 m RNA in group E was significantly higher than that in group C(0.953 ± 0.238 vs 0.631 ± 0.146,P < 0.05),which peaked at 2 h,and then decreased gradually.The expression of defensin-5 m RNA in group T was significantly lower than that in group E(0.487 ± 0.149 vs 0.758 ± 0.160,P < 0.05) apparently in 24 h.The expression of defensin-5 m RNA at 2 h in group T was significantly higher than that in group C(0.824 ± 0.158 vs 0.631 ± 0.146,P < 0.05).CONCLUSION:MPO and defensin-5 m RNA increase in preweaning rats with LPS-induced intestinal injury.Bifidobacterium protects the gut by inhibiting MPOactivity,not by increasing defensin-5 secretion.
AIM: To investigate the protective effect of bifidobacterium in endotoxin-induced intestinal injury in preweaning rats. METHODS: Preweaning rats were randomly divided into three groups (n = 40 for each): a control group (groupC), a model group ) and a treatment group (group T) .Both groups E and T were intraperitoneally injected with lipopolysaccharide (LPS) at a dose of 5 mg / kg in normal saline), and group T was intragastrically administrated with bifidobacterium suspension (2.0 × 109 CFU / m L, 0.5 m L each time, twice a day, until the end of the experiment) 7 days before LPS administration. Group C was intraperitoneally injected with normal saline. After intraperitoneal injection and intragastric administration, the rats were placed back to the initial cage to receive breast feeding. The rats were killed at 2,6,12,24 or 72 h, respectively, after endotoxin or physiological saline injection to collect serum and ileal tissue samples. Myeloperoxidase (MPO) contents in serum and ileum were detected at different times, and expression of ileal defensin-5 m RNA was evaluated by reverse transcription-polymerase chain reaction.RESULTS: Serum and ileal MPO contents in group E were significantly higher than those in group C (serum contents: 107.50 ± 17.70 vs 157.14 ± 24.67, P <0.05; Ileal contents: 1.03 ± 0.21 vs 1.57 ± 0.33, P <0.05), which peaked at 12 h and 6 h, respectively.MPO contents in group T were significantly lower than those in group E (serum contents: 114.38 ± 24.56 vs 145.25 ± 23.62, P <0.05; ileal contents: 1.25 ± 0.24 vs 1.57 ± 0.33, P <0.05). The expression of defensin-5 m RNA in group E was significantly higher than that in group C 0.238 vs 0.631 ± 0.146, P <0.05), which peaked at 2 h, and then gradually decreased. The expression of defensin-5 m RNA in group T was significantly lower than that in group E (0.487 ± 0.149 vs 0.758 ± 0.160, P <0.05) apparently in 24 h. The expression of defensin-5 m RNA at 2 h in group T was significantly higher than that in group C (0.82 4 ± 0.158 vs0.631 ± 0.146, P <0.05) .CONCLUSION: MPO and defensin-5 m RNA increase in preweaning rats with LPS-induced intestinal injury. Bifidobacterium protects the gut by inhibiting MPO activity, not by increasing defensin-5 secretion.