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7．1．4．3 C3G对大鼠肝脏微粒体酶解脂质抗氧化活性肝脏微粒体膜含有高浓度多不饱和脂肪酸,极易氧化。有人用8周龄、重180-200g大鼠肝脏经均质等后,用缓冲液配成浓度为2 mg／L基料,将C3G等试样溶于甲醇后加入基料,使浓度为50和100 μmol／L,然后将8 mmol／L ADP、0．4 mmol／L FeCl3、0．4 mmol／L EDTA和0．04 mmol／L NADPH加入50 mmol／L、pH 7．4 缓冲液,使最终浓度分别为2．0、0.、0．1和0．1 mmol／L,再在37℃下培养30分钟,之后用HPLC,尿素法测定所形成 MDA,结果见图14(n=3)。其中NADPH(还原型辅酶Ⅱ) 为微粒体酶解剂,FeCl3为催化剂,ADP和EDTA为螯合剂。从图中可知,C3G和Cy无论是50或100μmol／L,均有显著强抗氧化活性,比之同浓度Toc抗氧化活性更强。且Cy比之c3G和Toc具有更强抗氧化活性,即使浓度仅为50 μmol／L。据此,可认为Cy和C3G对 NADPH酶解脂质过氧化系统具有很强抗氧化活性。
7.1.4.3 C3G Lipid Antioxidant Activity in Rat Liver Microsomes Liver microsome membranes contain high concentrations of polyunsaturated fatty acids that are readily oxidized. Some people with 8 weeks of age, weight 180-200g rat liver homogenization, etc., with buffer dubbed a concentration of 2 mg / L base material, such as C3G dissolved in methanol after adding the base material to a concentration of 50 And 100 μmol / L, then adding 8 mmol / L ADP, 0.4 mmol / L FeCl3, 0.4 mmol / L EDTA and 0.04 mmol / L NADPH into 50 mmol / L pH 7.4 buffer, The final concentrations were 2.0, 0.1, 0.1, and 0.1 mmol / L, respectively, and incubated at 37 ° C for 30 minutes. The formed MDA was measured by HPLC and urea method. The results are shown in Fig. 14 (n = 3). Among them, NADPH (reduced coenzyme Ⅱ) is a microsomal digesting agent, FeCl3 is a catalyst, ADP and EDTA are chelating agents. It can be seen from the figure that both C3G and Cy have a strong anti-oxidant activity at 50 or 100 μmol / L, which is stronger than the same Toc anti-oxidant activity. Cy has stronger anti-oxidant activity than c3G and Toc, even at a concentration of 50 μmol / L. Accordingly, it can be considered that Cy and C3G have strong antioxidant activity against NADPH digested lipid peroxidation system.