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目的:研究阿司匹林对去势(卵巢切除)大鼠腰椎骨密度及微观结构的影响。方法:取48只3月龄SD雌性大鼠随机分为6组:去势组(OVX组)、对照组(Sham组)及4个阿司匹林治疗组(Aspirin组),每组8只。OVX组及Aspirin组采用卵巢切除法建立骨质疏松模型。去势后1周,阿司匹林治疗组剂量分别为2.25、4.46、8.92及26.75 mg/kg(A1、A2、A3及A4组),每天灌胃一次,OVX组及Sham组予同等量生理盐水灌胃。灌胃3个月后处死,剖取腰椎椎体,以双能X线吸收骨密度测量仪(DXA)和Micro-CT进行测量分析。结果:DXA分析结果显示:阿司匹林各剂量组BMD值较OVX组有统计学差异(P<0.01)。Micro-CT分析表明:与OVX组比较,阿司匹林各剂量组BV/TV、Tb.Th、Tb.N、BMD均显著性提高(P<0.01),BS/BV、Tb.Sp显著性降低(P<0.01),阿司匹林各剂量组BV/TV、BS/BV、Tb.Th、Tb.N、Tb.Sp、BMD与Sham组相比有统计学差异(P<0.01)。结论:阿司匹林可以改善去势大鼠骨小梁结构,增加骨质密度,对去势大鼠骨质疏松具有防治作用,其作用途径可能包括抑制骨吸收和刺激骨形成两方面。
Objective: To study the effect of aspirin on lumbar spine bone mineral density and microstructure in ovariectomized rats. Methods: Forty-eight 3-month-old female Sprague-Dawley rats were randomly divided into 6 groups: OVX group, sham group and aspirin group (Aspirin group), 8 in each group. OVX group and Aspirin group using ovariectomy to establish osteoporosis model. One week after castration, the doses of aspirin in treatment group were 2.25, 4.46, 8.92 and 26.75 mg / kg respectively (groups A1, A2, A3 and A4). The rats in OVX group and Sham group were given the same amount of normal saline . Three months after gavage, sacrifices were taken and lumbar vertebrae were dissected and measured by Dual Energy X-ray absorptiometry (DXA) and Micro-CT. Results: The results of DXA analysis showed that the BMD of aspirin in each dose group was significantly different from that in OVX group (P <0.01). Micro-CT analysis showed that BV / TV, Tb.Th, Tb.N and BMD were significantly increased (P <0.01), and BS / BV and Tb.Sp were significantly decreased in aspirin group compared with OVX group <0.01). The BV / TV, BS / BV, Tb.Th, Tb.N, Tb.Sp and BMD of each aspirin group were significantly different from those of Sham group (P <0.01). Conclusion: Aspirin can improve the structure of trabecular bone in ovariectomized rats, increase the bone density and prevent and cure osteoporosis in ovariectomized rats. Its mechanism of action may include inhibition of bone resorption and stimulation of bone formation.