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探讨经母体进入子代体内砷化物的形态及在肝和脑组织中的分布情况。小鼠从怀孕0 d起,以自由饮水方式暴露无机三价或五价砷,取其生后10 d仔鼠的肝和脑组织,采用氢化物发生-超低温捕集-原子吸收分光光度仪测定脏器中无机砷(iAs)、一甲基胂(MMA)、二甲基胂(DMA)、三甲基胂(TMA)的含量。仔鼠肝和脑组织中的DMA含量随染砷剂量的增加而增加,肝脏中DMA含量高于脑组织。肝和脑组织中iAs含量各染砷组与对照组比较差异无统计学意义。提示砷化物可以经母体进入子代体内,其形态应以DMA为主。
To explore the morphology and distribution of arsenic in the liver and brain tissue of the offspring entering the mother’s body. The mice were exposed to free trivalent or pentavalent arsenic from 0 d after pregnancy. The liver and brain tissues of the offspring of the 10 d postnatal mice were measured by hydride generation-ultra-low temperature capture-atomic absorption spectrophotometry The content of inorganic arsenic (iAs), monomethylarsine (MMA), dimethylarsine (DMA) and trimethylarsine (TMA) in organs were measured. The content of DMA in the liver and brain of pups increased with the increase of arsenic dose, and the content of DMA in the liver was higher than that in brain. There was no significant difference in the content of iAs in liver and brain between the groups with arsenic and control groups. Tip arsenide can enter the offspring through the mother body, its morphology should be dominated by DMA.