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目的优化空肠弯曲菌(CJS131)诱导系统性红斑狼疮(SLE)样小鼠模型的方法。方法小鼠随机分为5组,即正常对照组、卡介苗(BCG)对照组、结核分枝杆菌H37Ra对照组、CJS131+BCG模型组和CJS131+H37Ra模型组。在第0天免疫,在第14、21、42天加强免疫,在第36、54、61天分批称其体质量后处死。检测小鼠免疫器官指数、血清抗核抗体水平、血清总Ig G水平和肾组织病理损伤程度。结果不论采用含BCG还是H37Ra的弗氏完全佐剂(FCA),给予CJS131免疫的小鼠均有SLE样综合征的表现:血清抗核抗体及总Ig G水平升高,肾组织损伤。在动态检测中病变基本维持。结论 CJS131在对模型组小鼠的免疫诱导中起主要作用,以BCG或H37Ra作为FCA中采用的菌株都可引起病变,通过加强免疫可维持小鼠长期病变,优化后的模型可用于SLE治疗性药物的筛选。
Objective To optimize the method of inducing systemic lupus erythematosus (SLE) -like mouse model by Campylobacter jejuni (CJS131). Methods Mice were randomly divided into 5 groups: normal control group, BCG control group, Mycobacterium tuberculosis H37Ra control group, CJS131 + BCG model group and CJS131 + H37Ra model group. Immunized on day 0, booster immunizations on days 14, 21, and 42 and sacrificed on days 36, 54, and 61 for weight loss. Immune organ index, serum anti-nuclear antibody level, serum total Ig G level and renal tissue pathological damage were detected. Results All mice immunized with CJS131 exhibited SLE-like syndrome regardless of BCF or H37Ra complete Freund’s adjuvant (FCA). Serum anti-nuclear antibody and total Ig G levels were elevated and renal tissue was damaged. In the dynamic detection of the basic maintenance. Conclusion CJS131 plays a major role in immune induction in model mice. Both BCG or H37Ra can be used as a strain in FCA, which can cause long-term disease in mice. The optimized model can be used in the treatment of SLE Drug screening.