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目的研究北京市门头沟区流行麻疹病毒(Me V)的基因型别及特征。方法采集麻疹疑似患者咽拭子和尿液标本分离病毒,提取病毒RNA,逆转录PCR扩增病毒的N基因碳末端的634 bp的核苷酸片段,对扩增产物进行序列测定和比较分析。结果在发病的39例患者中,14例扩增到N基因片段,序列阳性率为35.9%,经过序列比对,均为H1基因型。本研究所获得的14株麻疹病毒H1基因亚型在亲缘关系进化树上与WHO推荐的H基因型代表株Hunan.China93-7/H1-H1c同属一个分支,核苷酸同源性为96.7%~97.9%,氨基酸同源性为95.5%~97.3%。结论 H1基因型麻疹病毒是门头沟区麻疹病毒的优势流行株,与北京市及全国麻疹病毒流行株无差异。
Objective To study genotypes and characteristics of MeV in Mentougou District of Beijing. Methods The virus was isolated from the throat swabs and urine samples from patients with suspected measles virus. The viral RNA was extracted and the 634 bp nucleotide fragment of the N terminal of the virus was amplified by reverse transcription polymerase chain reaction (RT). The amplified products were sequenced and compared. Results Among the 39 patients, 14 cases were amplified to N gene fragment, the positive rate was 35.9%. After sequence alignment, all were H1 genotypes. The 14 strains of measles virus H1 subtypes obtained in this study belong to the same branch of Hunan-China93-7 / H1-H1c, which is the WHO-recommended H genotype, on the phylogenetic tree. The nucleotide homology was 96.7% ~ 97.9%, amino acid homology is 95.5% ~ 97.3%. Conclusion The H1 genotype measles virus is the predominant epidemic strain of measles virus in Mentougou district and no difference with the measles virus epidemic strain in Beijing and the whole country.