当照射剂量小到足以使细胞在研究过程中存活的情况下,研究 DNA 分子损伤的形成和修复是很有意义的。虽然已经建立了几种测定哺乳动物细胞中DNA 单链断裂的灵敏方法,然而,试图测定在长期孵育后最终仍未修复的“临界”断裂仍然受现有方法的灵敏度所限制。 It is instructive to study the formation and repair of DNA molecular damage when the dose of irradiation is small enough to keep the cells alive during the course of the study. Although several sensitive methods for determining DNA single strand breaks in mammalian cells have been established, the “critical” cleavage that attempts to determine the ultimate unrecovered repair even after prolonged incubation is still limited by the sensitivity of current methods.