报告对出生后10～16d龄鸡初级听神经细胞（PAN）进行原代培养的方法；用倒置相差显微镜和扫描电镜，观察不同培养时间听神经元的生长发育过程与形态学特征。结果显示，培养PAN数量和形态以及存活时间，均可满足体外研究的要求，可作为多种体外研究的材料。表明PAN原代培养方法．扩大了培养材料的来源，为开展内耳体外研究提供了一种新的实验手段。 The method of primary culture of primary cultured human auditory neurons (PAN) from 10 to 16 days after birth was reported. The growth and morphological characteristics of auditory neurons at different culture time were observed by inverted phase contrast microscope and scanning electron microscopy. The results showed that the quantity and morphology of cultured PAN and survival time could meet the requirements of in vitro studies and could be used as materials for a variety of in vitro studies. Indicating the primary culture method of PAN. Expanding the sources of training materials and providing a new experimental method for carrying out in vitro studies of the inner ear.