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采用水囊引产眙儿(胎龄4～6个月),提取肝刺激因子(hHSS),用~3H—胸腺嘧啶核苷测其活性,观察到 hHSS 可使34%肝切除的大鼠对~3H—胸腺嘧啶核苷掺入再生肝 DNA 率明显增加。将 hHSS 预先注入半乳糖胺损伤的小鼠体内,结果观察到:hHSS 极明显地降低半乳糖胺中毒的死亡率(P<0.01),明显地抑制半乳糖胺所致的血清 GPT 和 GOT 的高水平。电镜检查表明,hHSS 可保护肝细胞的超微结构(线拉体、内质网等)免受半乳糖胺的损伤。这些结果证明,从人胎肝中可提取 hHSS,而 hHSS 具有保护肝脏免受肝毒剂—半乳糖胺的毒害作用。
Using water-sac to induce deafness (gestational age 4 to 6 months), extract hepatic stimulating factor (hHSS), measure its activity with ~3H-thymidine, observe that hHSS can make 34% of hepatectomized rats ~ 3H-thymidine incorporation significantly increased the rate of regenerating liver DNA. The hHSS was pre-infused into galactosamine-injured mice. As a result, it was observed that hHSS significantly reduced the mortality of galactosamine poisoning (P<0.01), and significantly inhibited the high serum GPT and GOT caused by galactosamine. Level. Electron microscopy showed that hHSS protects the ultrastructure of liver cells (strands, endoplasmic reticulum, etc.) from damage by galactosamine. These results demonstrate that hHSS can be extracted from human fetal liver, while hHSS protects the liver from the toxic effect of the hepatotoxic agent galactosamine.